contain B-lactam unit as an essential structure feature of its molecule (14). We have used schiff base condensation as the ring-closing step to synthesis oxazepine, diazepine, thio compound, the hetero atoms in there structure such as (S,N,O) explain variety of applications, antitumor, in the biological engineering and in other field of their specific structure(15). Schiff bases are anti-chemical good for some types of bacteria and fungi (16). Schiff base posses antituberior and anticancer activity(17) Gas chromatography (c-c) analysis of basic organic compounds (e.g. amines, basic drugs and azo dyes)is a challenge to the analyst, especially when the compounds are at low concentration(18) and these parathion mechanism depend on the selective phase, rate of carrier gas, temperature column(19) this technique is used to separate the numerous compounds, particularly organic compounds such as alcoholsand hydrocarbons aolastarate ketones and, Aldehyde etc(20)

Experimental Apparatus:-

All chemicals used (purity 99.98%), FT.IR-spectra: were recorded on Shimadzu 8300, KBr-disc, HNMR-spectra were recorded on Varian 300 MHZ spectrometer using TMS as an internal standard and elemental analysis (C.H.N)-elemental (Analyses system GmbH)-measurements were made at Department of Chemistry, Kashan University. Iran. The melting points were determined in open capillary tubes by electro thermal 9300 LTD, U.K.

Synthesis of Compounds [1,2]

A mixture of 4- nitro aniline (0,0 1mole) and 4-dimethyl amino benzaldehyde (0,01 mole) in the presence of absolute ethanol with drops of glacial acetic acid and reflux for (2h), the precipitate was filtered and dried with recrystallized to yield (86%) of compound [I], which (0.01mole) reacted with (0.01mole) of maleic anhydride respectively in presence of dry benzene with refluxing for(4h), the precipitates filtered, recrystallized from ethanol to produce(88%) of compound[2].

Synthesis of Compounds[3-5]

A mixture of(0.01mole) of compound [2] with(o.o1mole) of one of(hydro guandine, thiosemicarbazide, thiourea) were refluxed in presence of dry benzene for (4h), the precipitates filtered, recystallized from dry benzene to produce(80,82 and 180)% of compound[3],compound[4] and compound[5], respectively.

Synthesis of Compounds[6,7]

A mixture of (0,001 mole) of (dichloromethyl ) in the presence of KOH in absolute ethanol with drops of glacial acetic and reflux for (42h), (0,001 mole) of (benzoic acid) in the presence of (4N) hydro chloric acid and reflux for (36h), the precipitate was filtered and dried with recrystallized to yield(81,85)% of compound[6], compound[7], respectively.

Synthesis of Compound[8]

The reaction of compound[5] (0.01 mole) with (dichloro ethane) (0.01 mole) in the presence of KOH in absolute ethanol for (3h), resulted in the formation (82%) of compound [8]

Synthesis of Compounds[9-10]

Ethanolic mixture of(0.01 mole) of compound[4] with(0.01 mole) of oxalic acid and (0.001mole) of dichloro methane to were refluxed for (4h) yield(84,85)% of compound[9] and compound[10] respectively.

Synthesis of Compounds[11-14]

A mixture of (0.01 mole) of melamine with (o.o1 mole) of 4-dimethyl benzaldehyde in the presence of absolute ethanol with drops of glacial acetic acid and reflux for (4h), the precipitate was filtered and dried with recrystallised to yield (83%) of compound[11] which(0.01 mole) with (0.01 mole) of cystein respectively in presence of dry benzene with refluxing for (7h) the precipitate was filtered and dried with recrystallized to yield (80%) of compound[12], which (0.001 mole) with one of (0.001 mole) of (phthalic an hydride) in the presence of acetone with reflux for (4h) (0.003 mole) of (maliec acid) in the presence of absolute ethanol with droups of glacial acetic acid with reflux for(7h), The precipitates filtered, recrystallized to give (80,80) % of compound[13], compound[14] respectively.

Synthesis of Compounds[15,16]

(1 mole) of compound [13] with (2mole) of one of (oxalic acid, dichlorechane) were refluxed in presence of Ethanol and presence of K₂CO₃ for (7-8h), the precipitate was filtered and dried with recrystallized resulted in the formation (80,80)% of compound[15], compound[16] respectively.

Synthesis of Compounds[17-20]

A mixture of(0.03 mole) of 4-dimethylaminopemzaldehyd, with(0.01 mole) of melamine in the presence of absolute ethanol with drops of glacial acetic acid and reflux for (4h), the precipitate was filtered and dried with recrystallized to yield(82%) of compound [17], which of one (0.03 mole) of (3,5-dinitrosakylicacid, (0.004 mole) of o-thiol benzoic acid and alanine) were refluxed in presence of dry benzene for (9h), the precipitate was filtered and dried with recrystallized to yield (80,82,80)% of compound [18], compound[19] and compound[20], respectively.

Analysis of Compounds [2, 3, 4, 5, 9 and Mixture] by Gas Chromatography:

Preparation of diluted solutions (( concentration of 1ppm for vehicles)) of compounds [2, 3, 4, 5,9 and Mixture] after dissolved with ethanol was also attended by a mixture of compounds which prepared by mixing 10ml of each solution individually after shaking continuous., injected models by using a syringe(Hamilton) with a capacity of 10ml individually and then injected the mixture, and then install the measurement conditions through the use of nitrogen a gas flow of 25ml/min bus speeds and injection temperature was 25Cº degrees higher than the temperature separation column and then use a flame ionization detector is 50Cº higher than the temperatures of the column either column temperature programmed gradual increase of (90-160)Cº, taking into consideration the maximum temperature to avoid damage to the column. All data are shown in Table (1) and figures (11-16).

RESULTS AND DISCUSSION:

All synthesized compounds [1-20] were characterized by FT. IR- spectra, (C.H.N)- analysis, melting points and some of them by H.NMR-spectra]. FT.IR-spectra showed appearance absorption bends at (1604) cm^-1 due to (CH=N) imine group, absorption band at (1350) cm^-1 due to (C-NO₂) nitro group, absorption bends at (1298)cm^-1 due to (-N(CH₃)2) dimethyl amine in compound [A1]. Appearance of absorption band at (1689)cm^-1 due to carbonyl group (CO) lactam (CO-N), absorption band at (1714)cm^-1 due to carbonyl group (CO) of lactone (CO-O), absorption band at (2968)cm^-1 due to (C-H) aliphatic, absorption band at (1408, 1284)cm^-1 due to (C-NO₂) nitro group and (N(CH₃)2) in compound [A2]. Appearance of absorption band at (1691, 1691, 1698)cm^-1 due to (CO-N) lactam, absorption band at (3367, 3425, 3201, 3294, 3333, 3419)cm^-1 due to NH2, absorption band at (3041, 3100, 3030)cm^-1 due to (CH=CH) alkene, absorption band at (2929, 2933, 2999)cm^-1 due to (C-H) aliphatic, absorption band at (1284, 1250, 1298)cm^-1 due to (N(CH₃)2) in compounds [A3, A4, A5], absorption band at (1390, 1346)cm^-1 due to (C-NO2) nitro group in compounds [A3, A5], absorption band at (815)cm^-1 due to (C=S) in compound [A5]. Appearance of absorption band at (1680, 1689)cm^-1 due to (CO-N) of lactam, absorption band at (1608, 1606)cm^-1 due to (C=N) indo cycle of lactam, absorption band at (2910, 2929)cm^-1 due to (C-O) aliphatic, absorption band at (1355, 1384)cm^-1 due to (C-NO₂), absorption band at (1230, 1250)cm^-1 due to (N(CH3)2), absorption band at (3030, 3061)cm^-1 due to(CH=CH), in compound [A, A7], absorption band at (3282) cm^-1due to (NH) in compound [A6]. Appearance of absorption band at (1699)cm^-1 due to (CO-N) of lactam, absorption band at (3009)cm^-1 due to (CH=CH) of alkene, absorption band at (2897)cm^-1 due to (C-H) aliphatic, absorption band at (1380)cm^-1 due to (C-NO₂) nitro group, absorption band (1280)cm^-1 due to (N(CH₃)₂), absorption band at (798)cm^-1 due to (C=S) in compound [A8]. Appearance of absorption band at (1672, 1689)cm^-1 due to (CO-N)of amide, absorption band at (3304, 3321)cm^-1 due to (NH) of amide, absorption band at (3059, 3089)cm^-1 due to (CH=CH) of alkene, absorption band at (1220, 1284)cm^-1 due (N(CH₃)₂) in compounds [A9, A10]. Appearance of absorption band at (1618)cm^-1 due to (-CH=N-) of imine group, absorption band at (1608)cm^-1 due to (C=N) of indo cycle of melamine, absorption band at (3435,(3435, 3421))cm^-1 due to (NH₂) of amine, absorption band at (1240)cm^-1 due to (N(CH₃)₂) in compound [A11], which disappeared and other bands are appear at (1670)cm^-1 due to (CO-N) of amide and two bands at (1598, 2362)cm^-1 due to (C=N) of indo cycle of melamine, (SH) and band at (2972)cm^-1 due to (C-H) aliphatic, absorption band at (3230, 3365)cm^-1 due to (NH₂) and band at (2286)cm^-1 in compound [A12]. Appearance of absorption band at (1695, 1664)cm^-1 due to (CO-N) of amide, absorption band at (1600)cm^-1 due to (C=N) of indo cycle of melamine, absorption band at (2960, 2922)cm^-1 due to (C-H) aliphatic, absorption band at (1246, 1240)cm^-1 due to (N(CH₃)₂) in compounds [A13, A14] and band at (2362)cm^-1 due to (SH) and band at (3340, 3281)cm^-1 due to (NH₂) in compound [A13] and band at (1365)cm^-1 due to (CH₂-S) in compound [A14]. Appearance of absorption band at (1681, 1684)cm-1 due to (CO-N) of lactam , absorption band at (1604)cm-1 due to (C=N) indo cycle of melamine and bands at (2939, 2904)cm^-1 due to (C-H) aliphatic and bands at (1398, 1320)cm^-1 due to (CH₂-S) and bands at (1298, 1294)cm^-1 due to (N(CH₃)₂) in compounds [A15, A16]. Appearance of absorption bands at (1624, 1588, 1298)cm^-1 due to (CH=N) of imine group, (C=N) of indo cycle of melamine and (N(CH₃)₂) respectively in compound [A17]. Appearance of absorption band at (1688, 1680, 1662)cm^-1 due to (CO-N) of amide absorption band at (1595, 1608, 1597)cm^-1 due to (C=N) of indo cycle of melamine and bands at (1255, 1230, 1234)cm^-1 due to (N(CH₃)₂) in compounds [A18, A19, A20], absorption band at (1431)cm^-1 due to (CH-S) in compound [A19], absorption bands at (2904, 1371, 1165)cm^-1 due to (CH) aliphatic, (C-NO₂) and (C-O-C) ether group in compound [A18]. Absorption band at (2908, 3230)cm^-1 due to (C-H) aliphatic and (NH) amine group in compound [20]., all data are shown in Table (2) and figures (1-5).

Table (2) .FT.LR-data(cm-1) of compounds[1-20].

Others	(-CO-O-) of Lactone	(-CO-N-) of Lactam	(C=N) Imine group	Comp. No.
ـــــــــــــــــــــــــــــ	ــــــــــــ	ــــــــــــ	1604	[1]
(C-NO2):1458 ,(N(CH3)2): 1284, (C-H aliphatic): 29,8	1714	1689	ـــــــــــ	[2]
(C-NO2): 13%, (N(CH3)2): 1284, (C-H aliphatic): 2929, (CH=CH): 3041, (NH2): 3425, 3367	ــــــــــــ	1691	ـــــــــــ	[3]
(N(CH3)2):1250, (CH=CH): 3100, (C-H aliphatic): 2933, (NH2): 3201, 3294	ــــــــــــ	1691	ـــــــــــ	[4]
(C-NO2): 1346, (N(CH3)2): 1298, (C-H aliphatic): 2999, (CH=CH): 3030, (NH2):3333, 3419, (C=S): 815	ــــــــــــ	1698	ـــــــــــ	[5]
(C-NO2):1355, (N(CH3)2): 1230, (C-H aliphatic): 2910, (CH=CH): 3030, (NH): 3282, (C=N) indo cycle of diazitedine Four membering	ــــــــــــ	1680	ـــــــــــ	[6]
(C-NO2): 1384, (N(CH3)2): 1250,(C-H aliphatic): 2929, (CH=CH): 3061, ((C=N) of ando cycle of Four membring): 1606	ــــــــــــ	1689	ـــــــــــ	[7]
(C-NO2): 1380, (N(CH3)2): 1280,(C-H aliphatic): 2897, (CH=CH): 3009, (C=S): 798	ــــــــــــ	1699	ـــــــــــ	[8]
(C-NO2): 1356 (N(CH3)2): 1225, (CH=CH): 3059, ( (NH) of amide):3304	ــــــــــــ	1672	ـــــــــــ	[9]
(N(CH3)2): 1284, (CH=CH): 3089,(C-H aliphatic): 2968, (NH of amide)=3321	ــــــــــــ	1689	ـــــــــــ	[10]
(N(CG3)2): 1240, (NH2): 3435,3421, ( (C=N)of indo cycle of melamine)=1588	ــــــــــــ		1618	[11]
(N(CH3)2): 2286, (SH): 2362, (C-H aliphatic): 2972, (NH2): 3230, 3365, (C=N) in docycl of melamine	ــــــــــــ	1675	ـــــــــــ	[12]
(N(CH3)2): 1246, (SH): 2362, (CH aliphatic): 2960, (NH2): 3340, 3281, ( (C=N) of indo cycle of melamine): 1600	ــــــــــــ	1695	ـــــــــــ	[13]
(N(CH3)2): 1240, (CH2-S): 1365, (C-H aliphatic): 2939, ( (C=N) indo cycle of melamine):1604	ــــــــــــ	1664	ـــــــــــ	[14]
(N(CH3)2): 1298, (CH2-S): 1398, (C-H aliphatic): 2939(C=N) indo cycle of melamine	ــــــــــــ	1681	ـــــــــــ	[15]
(N(CH3)2): 1294, (CH2-S): 1320, (C-H aliphatic): 2904(C=N) indo cycle of melamine	ــــــــــــ	1684	ـــــــــــ	[16]
(N(CH3)2): 1298, (C=N) of indo cycle of melamine	ــــــــــــ	ــــــــــــ	1624	[17]
(C+NO2): 1371, (N(CH3)2)=1255, (C-H aliphatic): 2904( (C=N) indo cycle of melamine): 1595, (C-O-C): 11, 5	ــــــــــــ	1688	ـــــــــــ	[18]
(N(CH3)2): 1230, (CH-S): 1431, ( (C=N) of indo cycle of melamine): 1608	ــــــــــــ	1680	ـــــــــــ	[19]
(N-(CH3)2): 1234, (NH): 3230, (C-H aliphatic): 2908( (C=N) indo cycle of melamine): 1597	ــــــــــــ	1662	ـــــــــــ	[20]

Table( 3 ). H.NMR(.ɓ. ppm) of Some Compounds.

Other peaks	N(CH₃)₂	(N-CH-N) of alkene	(CO-CH=CH-CO) of alkene	Comp No.
(8,66) proton of(Iminegroup) (6,74-7,66) proton of phenyl rings	(2,99)	ـــــــــ	ــــــــــ	[1]
(9,63) proton of oxazepine ring (O-CH-N), (6,74-7,66) proton of phenyl rings.	(2,85)	ـــــــــ	(6,06) in oxazepine ring	[2]
(4,54)proton of (NH2-CS-), (6.88-7.65) proton of phenyl rings.	(2,98)	(2,46)	(6,71,6,73) in diazepine ring	[5]
(1.82, 2.06) proton of (CH2-N) of azirine cycle, (6,53,7.94) protons of phenyl rings.	(2,47)	(3,39)	(6,06,6,18) in diazepine ring	[8]
(9.63,10.42) protons of amide (-NH-CO-NH2), (6.74-7.65) protons of phenyl ring.	(2,99)	(2,47)	(2,25) in diazepine ring	[4]
(3.83) proton of amine(-NH-), (3.51) of (6.52-7.67) of phenyl rings.	(2,54)	(2,95)	(6,02,6,09) in diazepine	[6]
(5.91) of thiol group (SH), (3.39) of amine (NH), (1.82,1.74,1.37) of (CH-CH2), (6.53-7.65) phenyl rings.	(2,47)	(2,56)	ــــــــــ	[13]
(2.48,2.54) proton of (CO-CH2-CH2-CO), (6.52-7.67) proton of phenyl rings.	(2,35)	(3,88)	ــــــــــ	[14]
(3.38,3.51) proton of (N-CH-CH2-S), (3.51) proton of imidazolone (N-CH-N), (6.74-7.67) proton of phenyl rings.	(2,48)	ـــــــــ	ــــــــــ	[15]
(8.66) proton of Iimine group, (6.74-7.66) proton of phenyl rings.	(2,99)	ـــــــــ	ــــــــــ	[17]

Table (4). Physical properties and (C.H.N) analysis of compounds [1-20]

Found			M.P Co	M.F	Comp. No.
N %	H %	C %	M.P Co	M.F	Comp. No.
15.113	5.268	66.120	159	C₁₅H₁₅N₃O₂	[1]
11.209	4.398	62.005	180	C₁₉H₁₇N₃O₅	[2]
20.349	4.651	58.574	208	C₂₀H₂₀N₆O₄	[3]
19.432	4.401	56.383	198	C₂₀H₂₀N₆O₅	[4]
16.156	4.313	56.154	204	C₂₀H₁₉N₅O₄S	[5]
19.795	4.582	59.813	206	C₂₁H₂₀N₆O₄	[6]
16.843	4.274	65.315	214	C₂₇H₂₂N₆O₄	[7]
15.291	4.294	58.265	182	C₂₂H₂₁N₅O₄S	[8]
17.314	3.603	55.105	234	C₂₂H₁₈N₆O₇	[9]
19.114	4.371	57.482	202	C₂₁H₂₀N₆O₅	[10]
38.011	5.246	55.558	359	C₁₂H₁₅N₇	[11]
31.102	5.075	50.009	>360	C₁₅H₁₉N₈OS	[12]
22.624	4.315	56.153	>360	C₂₃H₂₂N₈O₃S	[13]
18.342	4.126	53.303	260	C₂₇H₂₆N₈O₇S	[14]
18.482	29.917	54.016	222	C₂₇H₁₈N₈O₇S	[15]
20.416	4.574	59.518	240	C₂₇H₂₆N₈O₃S	[16]
24.020	6.068	69.121	>360	C₃₀H₃₃N₉	[17]
18.115	3.147	35.086	>360	C₅₁H₃₉N₁₅O₁₈	[18]
13.352	4.593	65.891	>360	C₅₁H₄₅N₉O₃S₃	[19]
22.887	6.106	64.071	>360	C₃₉H₄₅N₁₂O₃	[20]

Fig (1) .FT.IR- Spectra of compound [4 ]

Fig (2) .FT.IR- Spectra of compound [8 ]

Fig (3) .FT.IR- Spectra of compound [ 11 ]

Fig (4) .FT.IR- Spectra of compound [ 12 ]

Fig (5) .FT.IR- Spectra of compound [ 17 ]

Fig (6) .1H.NMR- Spectra of compound [ 2 ]

Fig (7) .1H.NMR- Spectra of compound [ 10 ]

Fig (8) .1H.NMR- Spectra of compound [ 12 ]

Fig (9) .1H.NMR- Spectra of compound [ 13 ]

Fig (10) .1H.NMR- Spectra of compound [ 19 ]

Fig(11): Chromatogram of comp.[2]

Fig(12): Chromatogram of comp.[9]

Fig(13): Chromatogram of Mixture[2-5 ,9]

Fig(14): Chromatogram of comp.[5]

Fig(15): Chromatogram of comp.[4]

Fig(16): Chromatogram of comp.[3]

Polarity	Max operatory Temp.(M.O.T)	Column dimension	Formula	Composition	Liquid phase
Low polar	(300)	0.25mm I.D 0.12 Mm d.f		2,3-di-o-propionyl-6-t-butylsilyl derivative of ᵞ-cyclodextrin phase	DP5-25
Moderately polar	-20C؛-280C؛-(300)C؛	0.25mm I.D 0.25Mm d. f		14%Cyanopropyl phenyl poly siloxane	FS-BP10

INTRODUCTION: